Pdf plant regeneration from direct and indirect organogenesis. In direct organogenesis, the explant or the original part of the plant used to initiate the culture develops into the shoot and root directly. Somatic embryogenesis is an artificial process in which a plant or embryo is derived from a single somatic cell. Agar is generally used at a concentration of 612 gliter. Clivia miniata is an important indoor ornamental plant and has been reported to have medicinal value. Callus induction, direct and indirect organogenesis of. In tissue culture media, vitamin addition is not always common.
Most evidence suggests that direct embryo genesis proceeds from cells which were already embryogenically competent while they were. Plant development through organogenesis is the formation. Direct organogenesis from leaf and internode explants of. Th d f dh l d l f h l d lhe production of dihaploid plants from haploid cultures sh h hortens the time taken to achieve uniform homozygous lines and varieties 3.
Somatic embryos are formed from plant cells that are not normally involved in the development of embryos, i. Direct and indirect in vitro plant regeneration and the effect of. The sugarcane plantlets were obtained through direct axillary buds, apical meristem, and leaf whorl disk and indirect callus culture shoot. Plant tissue culture 5 for free study notes log on. Sorghum tissue culture and regeneration protocols mostly follow either organogenesis maheswari et al. Direct organogenesis the root explants harvested from the in vitro grown seedling are considered as an excellent material for culture initiation as compared to mature plant explant and the degree of contamination is very less. Tissue culture the maintenance or growth of tissue the maintenance or growth of tissue, in vitro, in a way that may allow differentiation and preservation of their function. Since embryo is an independent structure and does not have vascular supply, it is not supposed to be the plant organ. Organogenesis definition the formation of roots, shoots or flower buds from the cells in culture in manner similar to adventitious root or shoot formation in cuttings is called organogenesis organogenesis starts in the callus in response to the stimulation given by the chemicals in the medium. Merrill is one of the most important protein and oil rich crop in the world. Direct and indirect organogenesis of clivia miniata and.
Introduction taraxacum officinale dandelion is a herbal medicinal plant that has phytochemical properties when analyzed as a whole in view of roots, leaves, and flowers schutz et al. Plant tissue culture the growth or maintenance of plant cells, tissues, organs or whole plants in vitro. Crc press, boca raton, london, new york and washington d. This protocol may find application in citrus genetic improvement programs. Calli induction on ms agar medium supplemented with 2,4d 0. The present study aims at establishing alternate regeneration protocols for h. Literature survey revealed the occurrence of variability in tissue culture raised plants which did not produced truetotype plants from leaf explants. Materials and methods the experiments were carried out in the plant tissue culture laboratories, agriculture college, basrah university, basrah, iraq during 20112012. This research aimed to establish a protocol for optimal micropropagation of o. Borthakurbb a plant tissue culture laboratory, botanical survey of india, eastern regional centre, meghalaya793003, india b department of botany, gauhati university, assam, india. The production of direct buds or shoots from a tissue with no intervening callus stage is called direct organogenesis.
Furthermore, organogenesis causes the development of a plantlet with root and shoot while somatic embryogenesis leads to the formation of a somatic embryo. Jan 22, 2018 tolumnia genus equitant oncidium is a group of small orchids with vivid flower color. Organogenesis in vitro depends on the balance of auxin and cytokinins and the ability of the tissue to respond to phytohormones during culture. Direct organogenesis from hypocotylderived explants of annatto. Organogenesis is the formation of organs, either shoots or roots. The below mentioned article provides an overview on the organogenesis in plant tissue culture. Direct and indirect shoot organogenesis strategies for. Indirect shoot regeneration was initiated from callus culture using auxins and cytokinins, where compact globular meristemoids were produced. The endoderm of vertebrates produces tissue within the lungs, thyroid, and.
Using young leaves and shoot tips as explants, the regeneration frequencies were much higher than. Ultrastructural analysis of in vitro direct and indirect. Regenerationin plant cultures, a morphogenetic response to a stimulus that results in the products of organs, embryos, or whole plants. Taraxacum officinale, micropropagation, direct organogenesis, indirect organogenesis, in vitro germinated and wild plants.
Plant regeneration from direct and indirect organogenesis and. Organogenesis involves inducing the vegetative tissue to form organs shoot or root which eventually develop into a complete. It also has a great development potential as an ornamental plant with its attractive foliage and beautiful flowers. Rhaponticum carthamoides regeneration through direct and. This is the first report that direct and indirect organogenesis simultaneously. Direct and indirect method of plant regeneration from root. Torenia fournieri, the wishbone flower, is used for this laboratory exercise because the in vitro production of adventitious shoots from torenia is easy to control, seeds are easy to obtain, and. Explain in brief the history of plant tissue culture. The present invention relates in general to plant biotechnology. Based on the procedure, organogenesis has two categories. Somatic embryogenesis and direct as well as indirect shoot organogenesis are the most commonly used micropropagation techniques in plant tissue culture.
Direct organogenesis, embryogenesis, micro grafting. Vitamins are necessary compounds synthesized and utilized in plants. The main difference between organogenesis and somatic embryogenesis is that organogenesis is the induction of vegetative tissue to form organs whereas somatic embryogenesis is the induction of vegetative tissue to form an embryonic callus. Using young leaves and shoot tips as explants, the regeneration frequencies were much higher. Agara polysaccharide powder derived from algae used to gel a medium. Pdf direct and indirect organogenesis of clivia miniata and.
Direct organogenesis from cotyledons in cultivars of citrus. There is a large evidence base about the striking effect of genotype on plant organogenesis, even between closely related species, as it is in this case. Plant regeneration via direct somatic embryogenesis from leaf. Earlier studies have reported in vitro propagation of h. The present study aimed to investigate effect of growth regulators on callus induction, direct and indirect. A laboratory exercise on direct and indirect organogenesis from leaf explants is presented for students of plant tissue culture or plant propagation. Ericaceae through direct organogenesis from leaf, internode stem without axillary bud, and root explants of in vitro raised seedlings has been developed. Direct organogenesis from cold treated in vitro leaf. Apr 25, 2012 clivia miniata is an important indoor ornamental plant and has been reported to have medicinal value. Plant tissue culture terminology adventitiousdeveloping from unusual points of origin, such as shoot or root tissues, from callus or embryos, from sources other than zygotes. To avoid this, direct regeneration of the explants into plantlets can be tried.
Direct somatic embryogenesis and organogenesis pathway of plant regeneration can seldom occur. An efficient protocol for direct shoot organogenesis has been developed for the medicinal plant aerva lanata l. However, a major problem associated with in vitro culture is the possible occurrence of somaclonal variation through indirect organogenesis modi et al. Saurabh bhatia, tanmoy bera, in modern applications of plant biotechnology in pharmaceutical sciences, 2015. Callus cultures were induced from leaf, stem and cotyledonary explants of momordica charantia, at different auxin and cytokinin concentrations either in single or in combination in ms medium. A good example is the formation of somatic embryos. Direct and indirect organogenesis of jatropha temasek life. A comparative structural analysis of direct and indirect. In vitro organogenesis in the callus tissue derived from a small piece of plant tissue, isolated cells, isolated protoplasts, microspores etc.
Regeneration in plant cultures, a morphogenetic response to a stimulus that results in the products of organs, embryos, or whole plants. Somaclonal variationphenotypic variation, either genetic or epigenetic in origin. Dj eds plant tissue culture concepts and laboratory exercise, 2nd edn. Chapter 2 indirect organogenesis and histological analysis of l. Direct organogenesis from cotyledonary node explants of. During organogenesis, the three germ layers formed from gastrulation. High efficiency direct shoot organogenesis from leaf segments. Direct organogenesis, embryogenesis, micro grafting, meristem. Thousands of hybrids have been registered on royal horticulture society and showed great potential for ornamental plant market. Induction of organogenesis and somatic embryogenesis of.
The best callogenic response was observed from all. Direct organogenesis explant meristemoid primordium in many plants, subculturing of callus results in undesired variations of clones somaclonal variations. Pdf direct and indirect organogenesis of clivia miniata. Shoot organogenesis and plant regeneration from leaf. Healthy and ripe fruits were collected from local pummelo trees c. Effect of vitamins on in vitro organogenesis of plant. Chavan 1,2, 1department of botany, yashavantrao chavan institute of science, satara, india. Tolumnia genus equitant oncidium is a group of small orchids with vivid flower color.
In this regard, tissue culture techniques hold substantial promise. Glycine max regenerated by direct organogenesis, with shoot buds originating from cotyledonary node epidermal and subepidermal tissue, and bauhinia forficata link regenerated by indirect organogenesis. In the use of plant tissue culture technique for propagation, organogenesis and somatic embryogenesis are the two alternative pathways in addition to direct shoot culture. An efficient propagation and regeneration system via direct shoot organogenesis from leaf explant was established in this study. A callus phase is commonly included in tissue culture protocols. Regeneration was achieved from leaf segments of 20 days old in vitro plantlets raised on murashige and skoog ms medium containing 0. Plant tissue culturethe growth or maintenance of plant cells, tissues, organs or whole plants in vitro. Alpinia galanga has been propagated via tissue culture using micropropagation, direct and indirect organogenesis with rhizome buds as explants 23 4 5, but the success of these efforts. Organogenesis root, shoot and leaves but not embryo are the organs that are induced in plant tissue culture. A laboratory exercise to demonstrate direct and indirect. Flow cytometry confirmed similar ploidy level in the mother plant and plants regenerated through direct and indirect organogenesis. What is the difference between organogenesis and somatic. Plant tissue culture technique can be used as a solution for these problems and a proper alternative to produce diseasefree clones of ginger plant by direct and indirect organogenesis.
Jun 29, 2017 the key difference between organogenesis and somatic embryogenesis is that organogenesis is the formation of organs from embryo while somatic embryogenesis is the artificial formation of an embryo from somatic cells. Organogenesis is the phase of embryonic development that starts at the end of gastrulation and continues until birth. This lecture also explains about the environment required for plant. The below mentioned article provides a study note on organogenesis. In plant tissue culture, organogenesis is a process of differentiation by which plant organs like roots, shoots, buds etc. The key difference between organogenesis and somatic embryogenesis is that organogenesis is the formation of organs from embryo while somatic embryogenesis is the artificial formation of an embryo from somatic cells. Plant regeneration via direct somatic embryogenesis from. The study was conducted to analyze the effect of plant growth regulators on callogenesis and direct and indirect organogenesis of memordica charantia. Plants have been propagated by direct organogenesis for improved multiplication rates, production of transgenic plants, and most importantly for clonal propagation. However, the tissue culture and genetic transformation techniques that are well. In vitro plant regeneration from direct and indirect. Bd and bh, which developed direct organogenesis related events, as well as bt, which developed indirect organogenesis related events figure 1. Callus induction, direct and indirect organogenesis of ginger.
Genetic similarity values calculated on the basis of rapd and issr data among regenerated in vitro plants to the mother plant were ranged from 0. Protocol for rapid regeneration of the important medicinal plant gaultheria fragrantissima wall. Indirect shoot organogenesis and selection of somaclonal. This has been achieved in many plant species by altering the hormonal combination of the culture media. Direct and indirect organogenesis of clivia miniata and assessment of dna methylation changes in various regenerated plantlets article pdf available in plant cell reports 317. In culture, the explant develops into callus tissue in a medium containing either a particular concentration of auxin or a definite auxincytokinin ratio. The shoot tip about 5 mm long excised from the same 3weekold seedling of r. Following the establishment of the shoot culture, the shoots were subcultured regularly at 5weekintervals on ms medium, as described above. Chapter 2 indirect organogenesis and histological analysis. Organogenesis in vitro culture techniques plant biotechnology. No endosperm or seed coat is formed around a somatic embryo. Direct organogenesis bypasses the need for a callus phase.
Vitamins, in combination with other media constituents, have been shown to have direct and indirect effects on callus growth, somatic growth, rooting, and embryonic development. Citrus clementina, cotyledons, direct organogenesis, plant regeneration, tissue culture. Direct somatic embryogenesis and organogenesis pathway of. Rhaponticum carthamoides regeneration through direct and indirect organogenesis, molecular profiles and secondary metabolite production, plant cell, tissue and organ culture pctoc, 2015, pp. During the past four decades, plant tissue culture is used for the largescale micropropagation in commercial horticul ture and agriculture. The gesneriaceous perennial plant, lysionotus serratus, has been used in traditional chinese medicine. Direct organogenesis from leaf and internode explants of in vitro raised wintergreen plant gaultheria fragrantissima r. We developed an efficient in vitro micropropagation protocol from young leaves indirect organogenesis, young petals indirect organogenesis and shoot tips direct organogenesis of this plant. After 21 days of culture incubation, maximum number of shoot. Indirect organogenesis via callus phase was obtained from nodes and.
Saurabh bhatia, tanmoy bera, in modern applications of plant biotechnology in. Typically indirect organogenesis is more important for transgenic plant production. Direct and indirect plant regeneration from various explants african. Indirect organogenesis and histological analysis of 37 production of regenerated plant through indirect organogenesis is one possible way to contribute to genetic improvement, because there are some advantages of shoot regeneration from callus over direct shoot regeneration. This video lecture explains about the process of plant tissue culture including the stepbystep procedure of micro propagation. May 28, 2016 organogenesis definition the formation of roots, shoots or flower buds from the cells in culture in manner similar to adventitious root or shoot formation in cuttings is called organogenesis organogenesis starts in the callus in response to the stimulation given by the chemicals in the medium. Leaf explants taken from in vitrogrown plants were used to induce direct somatic embryogenesis. Rhizogenesis, caulogenesis and organogenesis abt2012026047n. In vitro organogenesis and plant regeneration from leaves of solanum candidum lindl, s.
Such medium is known as callus inducing or initiation medium. The aim of this study is to establish an efficient method for in vitro propagation. Direct shoot organogenesis was induced in immature somatic embryos, where cell division and protomeristem formation started in subepidermal and epidermal cell layers of hypocotyl. Pdf direct and indirect organogenesis of alpinia galanga. Till now, many laboratories show a great deal of interest to improve this crop by introducing value added agronomic traits. Direct organogenesis from leaf and internode explants of in. The biochemical engineer can grow plant cells in liquid culture on a large scalebioreactor 2. With plant cultures, this is the process by which the tissue or explant is first subdivide, then transferred into fresh culture medium. According to helal 2011 tissue culture can be employed as. May 25, 2016 direct organogenesis explant meristemoid primordium in many plants, subculturing of callus results in undesired variations of clones somaclonal variations. High efficiency direct shoot organogenesis from leaf. Difference between organogenesis and somatic embryogenesis.639 1383 699 889 1067 660 1062 600 1488 126 599 1122 105 887 1199 628 709 900 287 245 593 483 1040 580 1267 927 866 178 444 244 1474 449 1138 266 1279 1411 27 1125 299